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  • Title: Multiclass method for antimicrobial analysis in animal feeds by liquid chromatography-tandem mass spectrometry.
    Author: Borràs S, Companyó R, Guiteras J, Bosch J, Medina M, Termes S.
    Journal: Anal Bioanal Chem; 2013 Oct; 405(26):8475-86. PubMed ID: 23922055.
    Abstract:
    A rapid multiclass method that covers 50 antimicrobials from 13 different families in animal feeds was developed. Samples were extracted using a mixture of methanol, acetonitrile and a McIlvaine buffer combined with sonication. Feed extracts were simply diluted prior to injection, since the clean-up strategies that were tested, based on either solid-phase extraction or dispersive solid-phase extraction, were ineffective at minimizing matrix-related signal suppression/enhancement. Analysis was carried out by liquid chromatography coupled to tandem mass spectrometry using an electrospray ionization source operating in positive and negative modes. For the quantification, matrix-fortified standard calibration curves were used to compensate for matrix effects and losses in sample preparation. The method was validated in-house in pig, poultry and cattle feed matrices and showed satisfactory performance characteristics. Thus, the proposed approach was suitable for application in a routine high-throughput laboratory for the official control of feeds.
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