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  • Title: Co-infection with Arsenophonus nasoniae and Orientia tsutsugamushi in a traveler.
    Author: Edouard S, Subramanian G, Lefevre B, Dos Santos A, Pouedras P, Poinsignon Y, Mediannikov O, Raoult D.
    Journal: Vector Borne Zoonotic Dis; 2013 Aug; 13(8):565-71. PubMed ID: 23930974.
    Abstract:
    Here we report a case of co-infection with Orientia tsutsugamushi, the causative agent of scrub typhus, and Arsenophonus nasoniae in a woman with a rash and an eschar who returned from a trip to Southeast Asia. A. nasoniae was previously considered to be a secondary insect and tick endosymbiont of unknown pathogenicity in humans. We amplified both O. tsutsugamushi and A. nasoniae DNA from a skin eschar with qPCR, and a seroconversion for O. tsutsugamushi and A. nasoniae was observed with immunofluorescence assays and western blotting for this patient. And we used 2-D western blotting with an A. nasoniae antigen and polyclonal mouse anti-A. nasoniae antibodies produced in our laboratory to detect the specific antigenic A. nasoniae proteins.
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