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  • Title: Stage-specific expression of the lactate dehydrogenase-X gene in adult and developing mouse testes.
    Author: Jen J, Deschepper CF, Shackleford GM, Lee CY, Lau YF.
    Journal: Mol Reprod Dev; 1990 Jan; 25(1):14-21. PubMed ID: 2393579.
    Abstract:
    Lactate dehydrogenase-X (LDH-X), a glycolytic enzyme found only in mammalian testes and spermatozoa, is encoded by a single gene (Ldh-x) in the mouse haploid genome. Several studies have demonstrated that LDH-X is associated with germ cells at specific stages of development. We have examined the expression of the Ldh-x gene during mouse spermatogenesis and testis maturation using in situ mRNA hybridization and immunocytochemistry. The results showed that transcription and translation of the Ldh-x gene are initiated at the pachytene stage of germ cell differentiation. However, although the amount of LDH-X protein increased as the germ cells progressed to maturation, its mRNA level was greatly decreased. These observations were confirmed by Northern analysis of total RNA derived from fractionated spermatogenic cells and developing testes. Furthermore, Northern studies also indicated two sizes of Ldh-x transcripts among different populations of spermatogenic cells in mature mouse testis.
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