These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Identification of one or two α-globin gene deletions by isoelectric focusing electrophoresis.
    Author: Agarwal AM, Nussenzveig RH, Hoke C, Lorey TS, Greene DN.
    Journal: Am J Clin Pathol; 2013 Sep; 140(3):301-5. PubMed ID: 23955447.
    Abstract:
    OBJECTIVES: To investigate the utility of isoelectric focusing electrophoresis (IEF) for identifying patients with α-thalassemia, which results from the deletion of 1 or more of the α-globin genes. METHODS: Samples were selected based on their hemoglobin H (HbH) concentration observed using IEF. The samples were analyzed for the most common α-globin gene deletions using molecular analysis. RESULTS: α-Globin gene deletions corresponding to α-thalassemia trait or silent carrier were observed in all samples with the HbH less than 2% phenotype. The genotypes of the specimens with HbH greater than 5% were consistent with HbH disease, while the wild-type phenotype control samples showed a wild-type genotype. CONCLUSIONS: Low concentrations of HbH can be detected in a patient with 1 or 2 α-gene deletions using IEF.
    [Abstract] [Full Text] [Related] [New Search]