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  • Title: Structural and biochemical studies of a recombinant 25.5 kDa glutathione transferase of Taenia solium metacestode (rTs25GST1-1).
    Author: Roldan A, Torres-Rivera A, Landa A.
    Journal: Parasitol Res; 2013 Nov; 112(11):3865-72. PubMed ID: 23959386.
    Abstract:
    In this work, we studied a recombinant mu-class glutathione transferase of 25.5 kDa from Taenia solium metacestode (rTs25GST1-1) that follows Michaelis–Menten kinetics with 1-chloro-2,4-dinitrobenzene (CDNB). The kinetic parameters obtained for rTs25GST1-1 with CDNB and GSH were V(max) =12.04 μmol/min/mg and K(m)=1.38 mM, and V(max) =10.20 μmol/min/mg and K(m)=0.90, respectively. The optimal activity was found at pH 8 in the 37-40 °C temperature range. Circular dichroism studies for rTs25GST1-1 at different pH showed that it maintains a typical α-helix structure between pH 6.5-7.5, but loses it between pH 8 and 8.5. Thermal CD assays showed rTs25GST1-1 barely changed its secondary structure. Unfolding/refolding assays showed that rTs25GST1-1 retained its structure up to 40 °C without loss of its activity. Additionally, exposure of rTs25GST1-1 to cumene hydroperoxide did not produce significant changes in its structure and only affected 50% of its activity.
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