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  • Title: Cryopreservation of nematode Caenorhabditis elegans in the adult stage.
    Author: Hayashi M, Amino H, Kita K, Murase N.
    Journal: Cryo Letters; 2013; 34(4):388-95. PubMed ID: 23995406.
    Abstract:
    Cryopreservation of nematode Caenorhabditis elegans in the adult stage is of importance as the nematode is a powerful research model organism. In this study, we applied the protocol previously established for cryopreservation of the L4 nematode to the adult one, and achieved a survival rate of 84%. When ice seeding was induced with bacteria P. syringae directly added to the nematode suspension instead of using a pre-cooled steel sticking needle, comparable survival rate was obtained after thawing. Moreover, a simple freezing device composed of a polystyrene foam box surrounded by a Dewar vessel put in a deep freezer was developed for a practical use. This simple method obtained a survival rate of 69 ± 4% for the adult nematode after thawing.
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