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  • Title: [Purification of total flavonoids from the extracts of Thesium Chinese with macroporous adsorption resin].
    Author: Chu XQ, Hu YQ, Yue L.
    Journal: Zhong Yao Cai; 2013 Mar; 36(3):478-81. PubMed ID: 24010334.
    Abstract:
    OBJECTIVE: To optimize the process parameters for purifying total flavonoids from Thesium chinese with D101 macroporous adsorption resin. METHODS: Purification technology of total flavonoids from the extracts of Thesium chinese was investigated by dynamic adsorption method with the transfer rates and the purity of total flavonoids as indexes. RESULTS: The optimal purifying conditions were as follows: sample concentration was 2.259 mg/mL with a speed of 1 BV/h, eluent concentration was 70% alcohol with a speed of 1BV/h. After purification, the transfer rate of total flavonoids was 94.44% and the purity of total flavonoids achieved 12.45%, which was 4 times of that by coarse extraction (2.91%). CONCLUSION: The purification technology is simple, stable and can significantly improve the contents of total flavoids in extracts.
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