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  • Title: Restrictionless cloning.
    Author: Algire MA.
    Journal: Methods Enzymol; 2013; 529():125-34. PubMed ID: 24011040.
    Abstract:
    Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are contained within the insert to be cloned.
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