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  • Title: Steroid secretion by granulosa cells isolated from a woman with 17 alpha-hydroxylase deficiency.
    Author: Pariente C, Rabinovici J, Lunenfeld B, Rudak E, Dor J, Mashiach S, Levran D, Blankstein J, Geier A.
    Journal: J Clin Endocrinol Metab; 1990 Oct; 71(4):984-7. PubMed ID: 2401721.
    Abstract:
    Steroid synthesis in luteal/granulosa cells harvested after follicular aspiration in a patient with 17 alpha-hydroxylase deficiency was investigated. Follicular stimulation with purified FSH after the suppression of ACTH and gonadotropin secretion by corticoid analogs and superactive GnRH agonist permitted ovum pick-up and the study of steroid synthesis in the obtained granulosa cells. Progesterone synthesis was elevated while 17 alpha-hydroxy progesterone, testosterone, androstenedione, and estrogen production was minimal. Aromatase activity was retained in these cells, as demonstrated by the 100-fold increase in estrogen levels after the addition of androgens. Progesterone levels in follicular fluids were in the normal range, while estrogen, 17 alpha-hydroxy progesterone, and androgen levels were minimal. These results provide in vitro evidence of 17 alpha-hydroxylase deficiency in the granulosa cells of this patient.
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