These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Inhibition of otopathogenic biofilms by organoselenium-coated tympanostomy tubes.
    Author: Wang JC, Tran PL, Hanes R, Cordero J, Marchbanks J, Reid TW, Colmer-Hamood JA, Hamood AN.
    Journal: JAMA Otolaryngol Head Neck Surg; 2013 Oct; 139(10):1009-16. PubMed ID: 24030785.
    Abstract:
    IMPORTANCE: Tube occlusion and post-tympanostomy tube otorrhea (PTTO) are 2 major sequelae of tympanostomy tube placement. Plugging negates the function of the tympanostomy tubes and, along with chronic PTTO, can be financially burdensome owing to repeated surgical procedures and additional treatments. OBJECTIVE: To investigate the effectiveness of an organoselenium (OSe) coating on Donaldson tympanostomy tubes in inhibiting biofilm formation on the tympanostomy tubes. DESIGN: In vitro microbiologic study; all experiments were performed in a Texas Tech University Health Sciences Center basic sciences laboratory. INTERVENTIONS: Inhibition of biofilm formation was investigated by incubating OSe-coated vs uncoated (control) tympanostomy tubes in a nutrient broth containing either Staphylococcus aureus (Sa) expressing green fluorescent protein (GFP), nontypeable Haemophilus influenzae (NTHi) expressing GFP, or Moraxella catarrhalis (Mc) for 48 hours at 37 °C. All biofilms were quantified via colony-forming unit (CFU) assays. The Sa and NTHi biofilms were visualized using confocal laser-scanning microscopy (CLSM) and analyzed using the COMSTAT program. MAIN OUTCOMES AND MEASURES: The CFU assays, CLSM, and COMSTAT analysis revealed that compared with uncoated control tympanostomy tubes, OSe-coated tympanostomy tubes are able to inhibit Sa, NTHi, and Mc biofilm formation. RESULTS: The Sa and NTHi developed thick mature biofilms containing considerable biomass on uncoated tympanostomy tubes as determined by CLSM and COMSTAT analysis, while the OSe coating on the tympanostomy tubes drastically inhibited biofilm formation by Sa and NTHi. Quantitative CFU analysis revealed that this reduction in biofilm formation was significant, 6 logs for Sa (P < .001) and 4 logs for NTHi (P = .02). OSe coating also inhibited biofilm formation by Mc with a 4.5-log reduction (P < .001). CONCLUSIONS AND RELEVANCE: The OSe coating is a potential long-lasting agent to prevent biofilm development on tympanostomy tubes by otopathogens.
    [Abstract] [Full Text] [Related] [New Search]