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  • Title: Toxic mechanisms of 3-monochloropropane-1,2-diol on progesterone production in R2C rat leydig cells.
    Author: Sun J, Bai S, Bai W, Zou F, Zhang L, Su Z, Zhang Q, Ou S, Huang Y.
    Journal: J Agric Food Chem; 2013 Oct 16; 61(41):9955-60. PubMed ID: 24040863.
    Abstract:
    3-Monochloropropane-1,2-diol (3-MCPD) is a well-known food processing contaminant that has been shown to impede the male reproductive function. However, its mechanism of action remains to be elucidated. In this study, the effects of 3-MCPD on progesterone production were investigated using R2C Leydig cells. 3-MCPD caused concentration-dependent inhibition of cell viability at the IC25, IC50, and IC75 levels of 1.027, 1.802, and 3.160 mM, respectively. Single cell gel/comet assay and atomic force microscopy assay showed that 3-MCPD significantly induced early apoptosis. In addition, 3-MCPD significantly reduced progesterone production by reducing the expression of cytochrome P450 side-chain cleavage enzyme, steroidogenic acute regulatory protein, and 3β-hydroxysteroid dehydrogenase in R2C cells. The change in steroidogenic acute regulatory protein expression was highly consistent with progesterone production. Furthermore, the mitochondrial membrane potential and cAMP significantly decreased.
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