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  • Title: Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells.
    Author: Yamauchi D, Minamikawa T.
    Journal: FEBS Lett; 1990 Jan 15; 260(1):127-30. PubMed ID: 2404793.
    Abstract:
    We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata. The sequence covers the whole transcribed region as well as the 5'- and 3'-untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G-50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.
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