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  • Title: Production and secretion of a heterologous protein by turnip hairy roots with superiority over tobacco hairy roots.
    Author: Huet Y, Ekouna JP, Caron A, Mezreb K, Boitel-Conti M, Guerineau F.
    Journal: Biotechnol Lett; 2014 Jan; 36(1):181-90. PubMed ID: 24078130.
    Abstract:
    A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were constructed. One cassette was used to express the green fluorescent protein (GFP)-encoding gene in hairy roots grown in flasks. A stable and fast-growing hairy root line secreted GFP at >120 mg/l culture medium. GFP represented 60 % of the total soluble proteins in the culture medium. Turnip hairy roots retained sustainable growth and stable GFP production over 3 years. These results were superior to those obtained using tobacco hairy roots.
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