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  • Title: A highly selective ratiometric visual and red-emitting fluorescent dual-channel probe for imaging fluoride anions in living cells.
    Author: Zhu B, Kan H, Liu J, Liu H, Wei Q, Du B.
    Journal: Biosens Bioelectron; 2014 Feb 15; 52():298-303. PubMed ID: 24080208.
    Abstract:
    Recently, growing attention has been paid to the accurate determination of fluoride anion (F(-)) in the environment and living systems for its toxicity and biological function investigation. In this paper, we developed a ratiometric visual and red-emitting fluorescent dual-channel probe (1) employed Si-O bond as a highly selective recognition receptor for imaging F(-) in living cells. Probe 1 possesses a potential internal charge transfer (ICT) structure, and displays a large (158 nm) red-shifted absorption spectrum and the color changes from yellow to blue upon addition of F(-) in the aqueous solution. In addition, probe 1 can be used to detect F(-) quantitatively by the ratiometric absorption and turn-on fluorescence spectroscopy methods with excellent sensitivity. Finally, the results of its application to bioimaging of F(-) in living cells show that probe 1 would be of great benefit to biomedical researchers for investigating the effects of fluoride in biological systems.
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