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  • Title: Application of monoclonal antibodies and genetically-engineered hybrid B-subunit proteins to the analysis of the cholera/coli enterotoxin family.
    Author: Finkelstein RA, Burks MF, Rieke LC, McDonald RJ, Browne SK, Dallas WS.
    Journal: Dev Biol Stand; 1985; 59():51-62. PubMed ID: 2408946.
    Abstract:
    Single amino acid substitutions, introduced by genetic engineering, significantly modify the behavior of the B-subunits of the cholera/coli enterotoxin family in SDS-PAGE and also markedly affect the reactivity of the proteins with mouse hybridoma-derived monoclonal antibodies raised against H-LT. The results indicate that single amino acids play an important role in defining epitopes in these proteins.
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