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  • Title: Simultaneous screening analysis of 3-methyl-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid residues in edible animal tissues by a competitive indirect immunoassay.
    Author: Jiang W, Beier RC, Wang Z, Wu Y, Shen J.
    Journal: J Agric Food Chem; 2013 Oct 23; 61(42):10018-25. PubMed ID: 24090030.
    Abstract:
    Immunoassays contribute greatly to food safety. Yet there are no reported immunoassays that simultaneously detect MQCA and QCA, the marker residues for olaquindox and carbadox, respectively. Here, a broad-specificity mAb was successfully produced, and the mAb showed good cross-reactivity with both MQCA and QCA, having IC50 values in buffer of 4.8 and 9.6 ng/mL, respectively. The calibration curves ranged from 0.3 to 81 μg/kg. The average recoveries ranged from 76% to 108% at different spiked levels (2, 4, and 8 μg/kg for MQCA; and 4, 10, and 20 μg/kg for QCA), and the intra-/interday coefficients of variation were 4.2-13.3%. The limits of detection of MQCA and QCA in chicken, fish, pork, and shrimp were 1.76, 1.32, 1.90, and 1.18 μg/kg, respectively. This method was verified by LC-MS/MS, with a correlation coefficient above 0.98. The immunoassay was rapid and reliable for simultaneous screening analysis of MQCA and QCA residues.
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