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  • Title: Immunologic detection of a protein homologous to chicken progesterone receptor B subunit.
    Author: Peleg S, Schrader WT, Edwards DP, McGuire WL, O'Malley BW.
    Journal: J Biol Chem; 1985 Jul 15; 260(14):8492-501. PubMed ID: 2409088.
    Abstract:
    A monoclonal antibody described previously by us (Edwards, D. P., Weigel, N. L., Schrader, W. T., O'Malley, B. W., and McGuire, W. L. (1984) Biochemistry 23, 4427-4435) was used to study progesterone receptor B subunits of chick and hen oviduct. We find that the antibody does not recognize the form of receptor B able to bind [3H]progesterone in vitro. Rather, it reacts exclusively with a homologous protein of the same molecular weight, termed B antigen. The antigen is present in both immature estrogen-treated chicks and in egg-laying hens. This antigen is indistinguishable from the hormone-binding receptor species (termed receptor B) as shown by peptide mapping techniques using either Staphylococcus aureus V8 protease or trypsin. The B antigen and the hormone binder can be resolved by ion-exchange chromatography. Sedimentation velocity data show that the two proteins are present in distinct, separable cytosolic entities. The functional relationship between the two proteins has not been established.
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