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  • Title: Comparison of gram stain, DNA probe, and culture for the identification of species of Mobiluncus in female genital specimens.
    Author: Roberts MC, Hillier SL, Schoenknecht FD, Holmes KK.
    Journal: J Infect Dis; 1985 Jul; 152(1):74-7. PubMed ID: 2409187.
    Abstract:
    The detection of species of Mobiluncus in female genital specimens by DNA probe (with whole-chromosomal bacterial DNA), culture, and gram stain were compared by using specimens obtained from hospital patients, college students, and women attending a sexually transmitted disease clinic. Culture purification and speciation required an average of 37 days to complete, whereas the DNA-probe assay required five days. Gram stain was also rapid but did not allow speciation. There was 100% correlation between species identification by DNA probe and by conventional biochemical tests. Gram stain alone detected 90% of the samples that were positive by any method, whereas culture detected 77%-83% and DNA probe detected 52%-83%.
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