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  • Title: Trehalose is not a universal solution for solid lipid nanoparticles freeze-drying.
    Author: Doktorovova S, Shegokar R, Fernandes L, Martins-Lopes P, Silva AM, Müller RH, Souto EB.
    Journal: Pharm Dev Technol; 2014 Dec; 19(8):922-9. PubMed ID: 24099511.
    Abstract:
    OBJECTIVE: To prepare stable and easy to handle formulation of solid lipid nanoparticles (SLNs) by freeze-drying with or without cryoprotectants, as appropriate. MATERIALS AND METHODS: SLNs were freeze-dried without cryoprotectants or with cryoprotectants in quantities selected by freeze-thaw test (sucrose, glucose) or literature search (trehalose, maltose). Appearance, re-dispersability and size distribution of re-dispersed samples were evaluated. RESULTS: SLN could be freeze-dried using 10% sucrose, trehalose or maltose. Trehalose was effective in protecting one of presented formulations that was already very stable on its own; its efficiency in protecting other two formulations was limited. DISCUSSION: Our results are in line with various reports of successful freeze-drying of SLN, but considering the stability of original dispersions, no improvement was achieved. CONCLUSION: We confirmed that trehalose is among the most suitable cryoprotectant for SLN, however it did not improve shelf-life of the most stable formulation.
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