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  • Title: 3D presentation of a neurotrophic factor for the regulation of neural progenitor cells.
    Author: Zhou K, Thouas G, Bernard C, Forsythe JS.
    Journal: Nanomedicine (Lond); 2014; 9(8):1239-51. PubMed ID: 24102327.
    Abstract:
    BACKGROUND: Adequate cell-scaffold interactions and neurotrophin support are essential factors for neural regeneration. AIM: To provide insight into the biofunctionalization of complex 3D scaffolds with nanoscale precision, as well as the effect of spatial distribution of brain-derived neurotrophic factor (BDNF) and its prolonged stimulation in combination with enhanced cell affinity of nanofibrous scaffolds on the survival/proliferation and neurite outgrowth. METHODS & MATERIALS: We developed a versatile approach using layer-by-layer self-assembly to incorporate cell adhesion and spatial representation of neurotrophic factors into complex nanofibrous scaffolds. RESULTS: Heparin/poly-L-lysine (PLL) polyelectrolyte multilayers (PEMs) were deposited on electrospun poly-ε-caprolatone nanofibers. Well-controlled amounts of BDNF were immobilized on the PEM-modified nanofibers. In addition, longer neurite outgrowth was observed in neural progenitor cells cultured on PLL-terminating PEM scaffolds. The immobilized BDNF on PLL-terminated PEM scaffolds resulted in significantly longer neurites and higher cell numbers (p < 0.01) compared with BDNF-free and BDNF-adsorbed PLL-terminating scaffolds. Interestingly, there was no upregulation of TrkB-FL, TrkB-T1 or GAP-43 mRNAs with immobilized BDNF in day 5 cultures. DISCUSSION & CONCLUSION: This work reinforces the importance of the combinatorial effects of biomaterial scaffold nanostructure and spatial presentation of neurotrophins in directing neural progenitor cell fates.
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