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  • Title: Action of DCCD on the H+/O stoichiometry of mitoplast cytochrome c oxidase.
    Author: Lehninger AL, Reynafarje B, Costa L.
    Journal: J Inorg Biochem; 1985; 23(3-4):335-40. PubMed ID: 2410565.
    Abstract:
    The mechanistic H+/O ejection stoichiometry of the cytochrome c oxidase reaction in rat liver mitoplasts is close to 4 at level flow when the reduced oxidase is pulsed with O2. Dicyclohexylcarbodiimide (DCCD) up to 30 nmol/mg protein fails to influence the rate of electron flow through the mitoplast oxidase, but inhibits H+ ejection. The inhibition of H+ ejection appears to be biphasic; ejection of 2-3 H+ per O is completely inhibited by very low DCCD, whereas inhibition of the remaining H+ ejection requires very much higher concentrations of DCCD. This effect suggests the occurrence of two types of H+ pumps in the native cytochrome oxidase of mitoplasts.
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