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  • Title: Steady-state fluorescence polarization/anisotropy for the study of protein interactions.
    Author: James NG, Jameson DM.
    Journal: Methods Mol Biol; 2014; 1076():29-42. PubMed ID: 24108621.
    Abstract:
    Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein-ligand complex.
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