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  • Title: Studies of monoclonal antibodies specific for major histocompatibility complex products of the rat. III. Production and characterization of syngeneic and allogeneic monoclonal anti-idiotypic antibodies to monoclonal alloantibodies.
    Author: Hemmi S, Fenner M, Binz H, Winterhalter K, Wigzell H.
    Journal: Scand J Immunol; 1985 Jun; 21(6):575-84. PubMed ID: 2410975.
    Abstract:
    Sixteen monoclonal anti-idiotypic antibodies were prepared against RT1 antigen-specific monoclonal antibodies by means of an inhibition assay. Three (110-3, 112-89, and 133-4) were produced against antibody 3-8C2 (19), three (178-60, 181-14, and 184-38) against antibody 11/23/5, and three (144-3, 144-33, and 146-31) against antibody 1-5A4. One monoclonal anti-idiotypic antibody (21-39) was directed against antibody 1-8D2 and six (CE1-3, CE2-1, CE7-2, CE8-46, CE9-35, and CE10-2) against antibody 3-12A1. The anti-idiotypic antibodies were all highly specific for the respective monoclonal antibody used for the induction of the anti-idiotype and did not cross-react with other monoclonal antibodies.
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