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  • Title: An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry.
    Author: Ormanns W, Schäffer R.
    Journal: Histochemistry; 1985; 82(5):421-4. PubMed ID: 2411696.
    Abstract:
    An avidin-biotin alkaline-phosphatase (ABAP) staining method has been developed for the labeling of tissue sections and cell smears. The introduction of alkaline phosphatase as a marker enzyme through an avidin bridge results in excellent immunocytochemical labeling of different antigens using poly- and monoclonal antibodies. This technique avoids problems with endogenous peroxidase activity that sometimes occur using peroxidase staining procedures. The introduction of a preformed avidin-biotin alkaline-phosphatase complex (ABAPC) makes the presented technique as simple to handle as the widely used avidin biotin-peroxidase complex method (ABC). The ABAPC technique could be combined with other enzymatic labelings for double immunoenzymatic staining.
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