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  • Title: [Synthetic medium for testing of susceptibility. II. Susceptibility of bacteria to sulphonamides (author's transl)].
    Author: Abdou MA.
    Journal: Zentralbl Bakteriol Orig A; 1975 Jan; 230(1):86-103. PubMed ID: 241178.
    Abstract:
    A comparative study of 9 media for testing the susceptibility of bacteria to 7 sulphonamides has been carried out. The evaluated culture-media included 4 "Mueller Hinton" media (MH), 4 others based on the formula of the "Diagnostic Sensitivity Test" agar base (D.S.T.) and a "Synthetic Medium" (SM) which has been developed in our laboratory. MH and D.S.T. culture-media have been supplied by 4 manufacturing companies in Europe and USA. A total number of 21 bacterial cultures have been employed. The included species belong to the gram negative genera Citrobacter, Escherichia, Klebsiella, Proteus, Salmonella, Serratia and Shigella, and to the gram positive genus Streptococcus. Sulphafurazole, sulphasomidine, sulphamethoxazole, sulphamethoxypyridazine, sulphadimethoxine, sulphaphenazole and sulphamethoxydiazine have been investigated. SM proved to be clearly superior to the other 8 culture-media mentioned above. Just one MH afforded good results. The 3 other MH and 2 D.S.T. media were not quite satisfactory. The two remaining D.S.T. media were unsuitable. Moreover, the activity of the tested sulphonamides was not equally high against the concerned bacteria. The author discussed the significance of the inoculum-size and of competitive and noncompetitive sulphonamide-antagonists in commercial culture-media and the difficulties they raise by the interpretation of the results.
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