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  • Title: Survival and beneficial properties of lactic acid bacteria from raniculture subjected to freeze-drying and storage.
    Author: Montel Mendoza G, Pasteris SE, Otero MC, Fatima Nader-Macías ME.
    Journal: J Appl Microbiol; 2014 Jan; 116(1):157-66. PubMed ID: 24118924.
    Abstract:
    AIM: To evaluate the effect of freeze-drying and storage conditions on the viability and beneficial properties of lactic acid bacteria (LAB) for raniculture. METHODS AND RESULTS: Lactococcus lactis CRL 1584, L. lactis CRL 1827, Lactococcus garvieae CRL 1828 and Lactobacillus plantarum CRL 1606 viability under different conditions was studied. 10% lactose and 5% skim milk + 5% lactose were excellent lyoprotectants, but 5% skim milk + 5% lactose and whey protein concentrated (WPC) or WPC + sugars were the lower cost lyoprotective options. The effect of temperature depended on both lyoprotectants and storage time. Thus, for Lactococcus, skim milk, skim milk + sucrose and WPC + sucrose were selected for lyophilization and storage at 4°C and skim milk + lactose for 25°C. For Lact. plantarum CRL 1606, the best lyoprotectants for lyophilization and storage at 4°C were milk + sugars and WPS + sucrose and, at 25°C, skim milk + sucrose. CONCLUSIONS: Lactic acid bacteria viability after freeze-drying was strain-specific and depended on the lyoprotectant used. Highest viability was obtained when stored at 4°C, and the beneficial properties remained stable for 18 months independently of storage temperature. SIGNIFICANCE AND IMPACT OF THE STUDY: The studies reported for the first time in this work are of primary interest to obtain dried bacteria to be included in beneficial products for raniculture.
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