These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Differentiation-specific regulation of Schwann cell expression of the major myelin glycoprotein.
    Author: Poduslo JF, Windebank AJ.
    Journal: Proc Natl Acad Sci U S A; 1985 Sep; 82(17):5987-91. PubMed ID: 2412226.
    Abstract:
    Previous experiments demonstrated that Schwann cells from permanently transected sciatic nerves of adult rats synthesize basal levels of the major myelin glycoprotein (P0). This denervated preparation at 35 days after transection was characterized by the absence of both axons and myelin assembly. The present investigation demonstrates that production of P0 continues after culture of the Schwann cells as endoneurial slices for 14 days. Thus, the level of differentiation is unchanged in culture even though only basal levels of P0 are produced and post-translational processing is incomplete. In contrast, Schwann cells from 4-day-old rat sciatic nerves actively synthesized P0 and assembled myelin membrane; however, after only 4 days in culture biosynthesis of P0 ceased. Because the same culture conditions and precursor incorporation procedures were used for both neonatal and transected nerves, it is proposed that neonatal Schwann cells in culture return to a progenitor state that is not capable of P0 gene expression. This comparison, both before and after culture, of neonatal Schwann cells that are programmed to myelinate and Schwann cells from the adult transected nerve that were in a myelin-maintaining mode provides a useful model for investigating the mechanisms by which differential gene expression is controlled. These results confirm that axons are not necessary for specifying P0 gene expression by Schwann cells from the adult transected nerves. The role that axons play in controlling P0 gene expression by neonatal Schwann cells in culture, however, has yet to be determined. It is concluded that the differentiation and maturation of Schwann cells is multistage process that allows the sequential production of specific gene products.
    [Abstract] [Full Text] [Related] [New Search]