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  • Title: Characteristics of Sendai virus RNA transcriptive complex formed in the cytoplasm of infected ascites cells.
    Author: Vladimirtseva EA, Bakunts KA, Lebedeva EN, Bukrinskaya AG.
    Journal: Acta Virol; 1975 Sep; 19(5):374-80. PubMed ID: 241240.
    Abstract:
    Virus-specific structures with sedimentation coefficients of 250-300, 200 and 150 S were isolated from the polysome fraction of Sendai virus-infected Ahrlich ascitic carcinoma cells treated with cycloheximide, at early stages of infection (1.5 to 2 hours after inoculation). All these 3 types of structure contained both parental and newly synthesized viral RNA. RNA extracted from these structures consisted of 2 components sedimenting in sucrose density gradients in the zones of 50-70 and 35-40 S. Both components contained parental and newly synthesized RNA and were partially resistant to ribonuclease. RNA extracted from rapidly sedimenting structures (250-300 S) contained mainly the 50-70 S component; RNA recovered from 200 S structures contained the 35-40 S component. By analogy with reported data, the isolated forms of RNA have been characterized as transcriptive intermediates.
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