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  • Title: Target-induced electronic switch for ultrasensitive detection of Pb2+ based on three dimensionally ordered macroporous Au-Pd bimetallic electrode.
    Author: Chen X, Tian R, Zhang Q, Yao C.
    Journal: Biosens Bioelectron; 2014 Mar 15; 53():90-8. PubMed ID: 24125757.
    Abstract:
    A novel strategy for selective and sensitive amperometric detections of Pb(2+) was proposed based on the three dimensional ordered macroporous (3DOM) Au-Pd bimetallic electrode and target-induced methylene blue-single walled carbon nanotubes (MB-SWCNTs) as signal reporter. A DNA biosensor was fabricated by immobilizing capture probe DNA on the 3DOM Au-Pd bimetallic electrode, which further hybridized with the reporter DNA loaded on the MB-SWCNTs adduct upon the exposure of Pb(2+), inducing measurable electrochemical signal. Due to the dramatic signal amplification by the enhanced immobilization of DNA on the surface of 3DOM Au-Pd bimetallic electrode and MB-SWCNTs, coupling the low background signal produced by blank solution, ultra-low level (1 × 10(-19)M) of Pb(2+) could be detected. Under the optimal conditions, the electrochemical signal of the MB increased with the increasing Pb(2+) concentration, exhibiting a linear response in the range of 1 × 10(-17)-1 × 10(-4)M. Furthermore, with the application of Pb(2+) dependent DNAzyme, the proposed sensing system demonstrated high selectivity. This work represented a promising potential for on-site testing Pb(2+) in real drinking water and serum sample analysis.
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