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  • Title: Biphasic progesterone synthesis by the hamster preovulatory follicle in vitro.
    Author: Makris A, Ryan K.
    Journal: Endocr Res; 1985; 11(1-2):1-15. PubMed ID: 2412813.
    Abstract:
    In vitro time course studies of progesterone and protein synthesis by hamster preovulatory follicles (harvested prior to the proestrous gonadotropin surge) were done. LH appears to stimulate progesterone synthesis in 2 phases. The first phase lasts 0 to 4 or 5 hr and is not inhibited by either puromycin or cycloheximide. The second phase (apparent by 4-5 hr) is distinguished from the first phase by its inhibition by puromycin and cycloheximide, and by the differential dose response of dibutyryl cyclic AMP on the respective 2 phases. Incorporation of a H-labelled mixture of amino acids into protein is not seen in LH-stimulated follicles in 2 hr incubations but is apparent by 4 hr. The LH-stimulated protein synthesis is inhibited by puromycin. The results indicate that although LH stimulates both phases of progesterone synthesis, the mechanisms may be different for each phase.
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