These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Synthesis of 4-nitrophenyl caffeate and its use in assays of caffeoyl esterases.
    Author: Chyba A, Mastihuba V, Mastihubová M.
    Journal: Anal Biochem; 2014 Jan 15; 445():49-53. PubMed ID: 24135652.
    Abstract:
    We have prepared 4-nitrophenyl caffeate by a combination of standard procedures of organic synthesis and enzymatic deacetylation. Based on hydrolysis of 4-nitrophenyl caffeate, a convenient spectrophotometric assay was developed for specific monitoring of caffeoyl esterase. The method is fast and easy to perform, and it requires no expensive equipment. Its reliability was tested on eight enzyme preparations comprising various combinations of caffeoyl, feruloyl, and acetyl esterase as well as protease activities.
    [Abstract] [Full Text] [Related] [New Search]