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  • Title: [Modification of a method of cell enucleation using cytochalasin B and the study of cytoplast viability].
    Author: Neverova ME, Kirsanova LA, Chentsov IuS.
    Journal: Tsitologiia; 1985 Jul; 27(7):785-91. PubMed ID: 2413596.
    Abstract:
    The modification of Prescott's (Prescott et al., 1972) method of enucleation in vitro was described. A special teflon chamber faciliatating the enucleation of monolayer cultured cells to produce cytoplasts and karyoplasts was constructed. Mouse L-cells were enucleated by exposing to cytochalasine B (10 gamma/ml) followed by centrifugation. The fraction of cells enucleated in the chamber was about 98%. The life time of cytoplasts in cultural medium after their enucleation was 48 hours (sometimes 56-72 hours) as tested by vital neutral red staining. The cytoplasts that survived were shown to accumulate large lysosomes, and the evidence of appearing ring-like fibrillar structures was provided using a simple technique of cytoskeleton observation under light microscope.
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