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  • Title: Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS.
    Author: Takahashi D, Li B, Nakayama T, Kawamura Y, Uemura M.
    Journal: Methods Mol Biol; 2014; 1072():481-98. PubMed ID: 24136542.
    Abstract:
    Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification.
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