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  • Title: Human inter-alpha-trypsin-inhibitor: characterization and partial nucleotide sequencing of a light chain-encoding cDNA.
    Author: Bourguignon J, Diarra-Mehrpour M, Sesboüé R, Frain M, Sala-Trepat JM, Martin JP, Salier JP.
    Journal: Biochem Biophys Res Commun; 1985 Sep 30; 131(3):1146-53. PubMed ID: 2413856.
    Abstract:
    A synthetic Inter-alpha-Trypsin-Inhibitor (ITI) -specific oligonucleotide probe was used to isolate a clone from a human liver cDNA library. The amino-acid sequence deduced from partial nucleotide sequencing of the corresponding cDNA insert perfectly matched a known ITI sequence, apart from an as yet unreported C-terminal dipeptide. Hybridization on Northern blots evidenced that this cDNA insert originated from an ITI light chain-encoding mRNA whose size was estimated to be 1 300 bases.
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