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  • Title: 16S rRNA PCR followed by restriction endonuclease digestion: a rapid approach for genus level identification of important enteric bacterial pathogens.
    Author: Vergis J, Negi M, Poharkar K, Das DP, Malik SV, Kumar A, Doijad SP, Barbuddhe SB, Rawool DB.
    Journal: J Microbiol Methods; 2013 Dec; 95(3):353-6. PubMed ID: 24140577.
    Abstract:
    The study describes a rapid approach for detection of common enteric bacterial pathogens, which involves partial amplification of the 16S rRNA gene by PCR using a colony from selective medium followed by restriction enzyme (RE) digestion using the EcoRI, HindIII and SalI enzymes. On the basis of RE digestion analysis different genera namely, Escherichia, Salmonella, Shigella, Vibrio, Campylobacter, Arcobacter, Yesinia and Listeria were differentiated.
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