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  • Title: E-assay concept: detection of bisphenol A with a label-free electrochemical competitive immunoassay.
    Author: Wang X, Reisberg S, Serradji N, Anquetin G, Pham MC, Wu W, Dong CZ, Piro B.
    Journal: Biosens Bioelectron; 2014 Mar 15; 53():214-9. PubMed ID: 24140871.
    Abstract:
    A label-free electrochemical immunosensor was developed by electropolymerization of N-(3-(4-(2-(4-hydroxyphenyl)propan-2-yl)phenoxy)propyl) 3-(5-hydroxy-1,4-dihydro-1,4-dioxonaphthalen-2(3)-yl)propionamide (JugBPA). By combination with an antibody directed to bisphenol A (αBPA), this conducting polymer-based biosensor can detect BPA directly with a limit of detection of 2pgmL(-1). Square wave voltammetry shows that the polymer film presents a current decrease upon anti-BPA binding and an opposite current increase upon BPA addition in solution. This electrochemical immunosensor (E-assay) also shows high selectivity towards closely related compounds (bisphenol A dimethacrylate, and dibutyl phthalate). The E-assay concept described here could be a promising tool for simple, low-cost and reagentless on-site environmental monitoring.
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