These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Structure and biological activity of polysomes stained with Coomassie blue.
    Author: Murthy MR, Hanna N, Bharucha AD, Charbonneau R, Viallard JL, Dastugue B.
    Journal: FEBS Lett; 1985 Oct 21; 191(1):131-5. PubMed ID: 2414125.
    Abstract:
    Polysomes prestained with Coomassie blue were fractionated on sucrose density gradients giving rise to visible bands corresponding to different size classes of aggregates. Coomassie blue staining enhanced the capacities of brain and liver polysomes to synthesize proteins in vitro, including the synthesis of neuron-specific enolase. This positive action of the dye was restricted to polysomes and was not manifested when mRNAs isolated from prestained polysomes were tested in in vitro translation or reverse transcription, indicating that the action of the dye consists in stabilization of polysomal structure.
    [Abstract] [Full Text] [Related] [New Search]