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  • Title: Evidence for negative control of cya transcription by cAMP and cAMP receptor protein in intact Escherichia coli cells.
    Author: Mori K, Aiba H.
    Journal: J Biol Chem; 1985 Nov 25; 260(27):14838-43. PubMed ID: 2414300.
    Abstract:
    The transcriptional regulation of cya by cAMP and its receptor protein (CRP) has been studied by S1 nuclease and RNA dot blot assays. The crp- Escherichia coli cells were shown to produce about 5-fold more cya mRNA than do the wild type cells. The effect of cAMP and CRP on the cya transcription was directly examined by introducing a crp plasmid into the cells and/or by adding cAMP exogenously. The levels of cya mRNA in crp+ cells decreased with increasing concentrations of cAMP in the growth medium. The repressive effect of cAMP on cya transcription was strongly enhanced in cells carrying a multicopy crp plasmid. These results indicate that the cya transcription is negatively regulated by cAMP-CRP complex in intact cells.
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