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  • Title: A study of insulin and glucagon secretion from adult rat pancreatic monolayer islets.
    Author: Ohgawara H, Tasaka Y, Machiyama E, Sakurai Y, Hirata Y.
    Journal: Tohoku J Exp Med; 1985 Sep; 147(1):15-20. PubMed ID: 2416096.
    Abstract:
    The present study confirms and extends previous observations that whole pancreatic islets form a monolayer culture in vitro. Our technique, using a medium containing 3-isobutyl-1-methylxanthine (IBMX), clearly demonstrated enzymatic disruption of the islets and cellular organization of isolated pancreatic islets. Insulin or glucagon secretion of monolayer culture was measured during incubation in a medium containing 5.5 mM D-glucose, then in 16.7 mM D-glucose, and finally in a combination of 16.7 mM D-glucose and IBMX, or of low glucose and 20 mM L-arginine. Clearly, such a technique might permit the recovery of collagenase-isolated pancreatic islets during the culture period and also an increase in glucose-induced insulin secretion and arginine stimulated glucagon secretion.
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