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  • Title: Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.
    Author: Chen HW, Zhang Z, Halsey ES, Guevara C, Canal E, Hall E, Maves R, Tilley DH, Kochel TJ, Ching WM.
    Journal: Am J Trop Med Hyg; 2013 Dec; 89(6):1088-94. PubMed ID: 24166046.
    Abstract:
    We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.
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