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  • Title: Ammoniacal silver staining of lymph node cells. II. Reactive follicular hyperplasia and its relationship with Hodgkin's disease.
    Author: Rengifo E, Quintero S, Marinello Z.
    Journal: Neoplasma; 1985; 32(6):741-4. PubMed ID: 2418374.
    Abstract:
    Lymph node biopsy specimens from 150 cases of reactive follicular hyperplasia, 52 cases of Hodgkin's disease and 30 cases of chronic lymphadenitis were studied. Clinical follow-up of cases for a period of two years at least was also considered. Formalin fixed and paraffin embedded tissues were stained with hematoxylin and eosin, and ammoniacal silver. Cytoplasmic accumulations of basic proteins were evidence in 30 out of 150 cases, but only 27 of these cases presented new lymph nodes with histological setting of Hodgkin's disease. The data suggest possible relationship between reactive follicular hyperplasia and Hodgkin's disease. However this relationship is not absolute as documented by two cases that being negative for the ammoniacal silver staining, were later diagnosed as Hodgkin's disease. Different types of atypical cells occurring in lymph nodes of reactive follicular hyperplasia were also studied. The potential use of ammoniacal silver staining in the early diagnosis of Hodgkin's disease is discussed.
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