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  • Title: Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.
    Author: Wen J, Li Z, He G, Xu S, Zhao B, Zhu X, Dong H, Ju J.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2013 Nov; 69(Pt 11):1227-30. PubMed ID: 24192355.
    Abstract:
    Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M Tris-HCl pH 8.0, 0.2 M LiSO4, 22%(w/v) PEG 3350. X-ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°.
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