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  • Title: Flow-cytometric determination of glutathione alterations in vital cells by o-phthaldialdehyde (OPT) staining.
    Author: Treumer J, Valet G.
    Journal: Exp Cell Res; 1986 Apr; 163(2):518-24. PubMed ID: 2420623.
    Abstract:
    A flow-cytometric method for the detection of changes of cellular glutathione content in vital cells is described. The reaction is based on formation of a fluorescent product between o-phthaldialdehyde (OPT) and reduced glutathione (GSH). OPT is a more GSH-specific dye than other thiol-specific dyes (e.g., bromobimanes), because it forms a cyclic compound with GSH. Changes of GSH induced by oxidation or thiol-blocking agents are visualized in vital cells after a 5-min staining at room temperature.
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