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  • Title: Structure of the dodecameric Yersinia enterocolitica secretin YscC and its trypsin-resistant core.
    Author: Kowal J, Chami M, Ringler P, Müller SA, Kudryashev M, Castaño-Díez D, Amstutz M, Cornelis GR, Stahlberg H, Engel A.
    Journal: Structure; 2013 Dec 03; 21(12):2152-61. PubMed ID: 24207124.
    Abstract:
    The type III secretion system machinery, also known as the injectisome, delivers bacterial effector proteins into eukaryotic cells during infection. The outer membrane YscC secretin is a major part of Yersinia enterocolitica's injectisome and is among the first components to assemble, solely assisted by its pilotin, YscW. We have determined the three-dimensional structures of the native complex and its protease-resistant core to 12 Å resolution by cryo-electron microscopy (cryo-EM) and show that YscC forms a dodecameric complex. Cryo-EM of YscC reconstituted into proteoliposomes defines the secretin's membrane-spanning region. Native YscC consists of an outer membrane ring connected via a thin cylindrical wall to a conical, periplasmic region that exposes N-terminal petals connected by flexible linkers. These petals harbor the binding site of YscD, a component of the inner membrane ring. A change in their orientation adapts the length of the YscC secretin and facilitates its interaction with YscD.
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