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  • Title: Synthesis of a myelin-like membrane by oligodendrocytes in culture.
    Author: Rome LH, Bullock PN, Chiappelli F, Cardwell M, Adinolfi AM, Swanson D.
    Journal: J Neurosci Res; 1986; 15(1):49-65. PubMed ID: 2421002.
    Abstract:
    We have prepared highly purified cultures of rat oligodendrocytes by a modification of the procedure of McCarthy and de Vellis [1980]. By utilizing a substratum derived from lysed glia and a calf serum-containing medium with a high concentration of transferrin, the oligodendrocyte cultures display a high degree of purity, the ability to survive several months of culture, and a striking ability to produce a myelin-like membrane. We have examined the production of this myelin-like membrane using immunocytochemical and biochemical probes as well as an extensive morphological examination at the electron microscopic level. The membrane appears to be produced in a similar developmental pattern to that observed in vivo and it has the structural characteristics of loosely packed central nervous system myelin.
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