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  • Title: [Recognition of excretory/secretory antigens of Anisakis type I and evolution of IgE in experimentally infected rats].
    Author: Gómez-Mateos M, Valero-López A, de la Rubia-Nieto T, Romero-López Mdel C, Díaz-Sáez V.
    Journal: Enferm Infecc Microbiol Clin; 2014 Oct; 32(8):491-6. PubMed ID: 24211134.
    Abstract:
    INTRODUCTION: Anisakis spp., during parasitism, release excretory-secretory antigens that, in contact with the human immune system, can trigger a hypersensitivity response mediated by IgE, causing various allergic symptoms. OBJECTIVES: To evaluate the IgE response in Wistar rats after infection with L3 larvae of the parasite Anisakis spp. METHODS: Some determining factors involved in the technique have been improved in this work, such as: the concentration of polyacrylamide used in the preparation of the gels, the antigen concentration used, and the temperature required for denaturation of proteins. RESULTS: Immune responses (Ag-Ab) observed by the immunoblotting technique showed a greater intensity with serum obtained after reinfection, which have recognized proteins that may correspond to the major antigen Ani s 1 and other polypeptides of interest in the diagnosis of human anisakiasis. CONCLUSION: This paper concludes that immunoblotting is a useful technique to detect IgE antibodies against Anisakis proteins.
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