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  • Title: [Preparation and characteristics of monoclonal antibodies against GTP-binding protein from the bovine retina].
    Author: Shamborant OG, Kolosov MI, Vasilov RG, Lipkin VM, Ovchinnikov IuA.
    Journal: Bioorg Khim; 1986 Mar; 12(3):309-15. PubMed ID: 2421734.
    Abstract:
    Five monoclonal antibodies against the native GTP-binding protein (transducin) from bovine retina have been prepared. By immunoblotting and immunoenzymatic analysis of the isolated alpha- and gamma-subunits of transducin and the beta gamma-subunit complex it was determined that two monoclonal antibodies A3G7 and A3C10 recognize linear antigenic determinants on the alpha-subunit, two other, A3E4 and 3B3, bound specifically to the gamma-subunit, and monoclonal antibodies 1C3 interact only with native transducin. Both antibodies against the alpha-subunit inhibited transducin GTPase activity, whereas antibodies A3E4, 3B3 and 1C3 did not affect it.
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