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  • Title: Denaturing gradient gel electrophoresis identifies genomic DNA polymorphism with high frequency in maize.
    Author: Riedel GE, Swanberg SL, Kuranda KD, Marquette K, Lapan P, Bledsoe P, Kennedy A, Lin BY.
    Journal: Theor Appl Genet; 1990 Jul; 80(1):1-10. PubMed ID: 24220803.
    Abstract:
    We have used denaturing gradient gel electrophoresis (DGGE) to identify genomic DNA polymorphism in maize (Zea mays L.). DGGE probes detect polymorphism in maize at a frequency comparable to the incidence of restriction fragment length polymorphism (RFLP). Probes identifying polymorphism were mapped to maize chromosome arms by utilizing DGGE and maize lines carrying B-A chromosomal translocations. The methods for library construction, probe screening, and genome analysis, described here for maize, can also be applied to the genomic analysis of other organisms.
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