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  • Title: [Semiautomatic method of measurement of serum 5'nucleotidase activity].
    Author: Hennon G, Plaquet R.
    Journal: Ann Biol Clin (Paris); 1975; 33(1):21-8. PubMed ID: 242242.
    Abstract:
    A semi-automated method for determining the 5'-nucleotidase activity in human serum for use with the Technicon Autoanalyzer I is described. Based upon the manual method of Persijn and Van Der Slik, adenosine formed by hydrolysis of 5'-AMP is deamined enzymatically and ammonia determined with the Berthelot reaction. The non specific alkaline phosphatases are inhibited by phenylphosphate. Concentrations of 5'-AMP and phenylphosphate are discussed. Nucleotidase activity is determined without dilution up to a value of 210 UI/liter. The precision is better than other manual or semi-automated methods in current use.
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