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  • Title: Nucleic acid staining with the methyl green-pyronin method. A comparison of the use of pure dyes and commercially available dyes.
    Author: Andersen AP, Lyon H, Jakobsen P, Høyer PE.
    Journal: Histochemistry; 1986; 84(3):279-80. PubMed ID: 2423483.
    Abstract:
    We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.
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